AmberGenMiralys™ MALDI Imaging

Next Generation Mass Spec Imaging

Verulam Scientific is pleased to work with AmberGen enablers of pioneering discoveries in the life sciences by solving key problems that limit progress in biotechnology and medicine. Their approach combines patented techniques in proteomics, mass spectrometry and light-based control of biomolecules to innovate in the fields of spatial biology, diagnostics and therapeutics.

Miralys MALDI-IHC imaging reagents have been widely adopted by the worldwide mass spectrometric imaging (MSI) community for high-plex, multiomic and multimodal spatial imaging of intact proteins and unlabelled small molecules in tissue on a mass spectrometer.

AmberGen’s Photocleavable Mass Tag (PCMT™) Technology

At the heart of AmberGen’s MALDI-IHC platform are its patented photocleavable mass-tags (PCMTs). These mass-tags act as barcodes, which when detected by a MALDI Mass Spectrometer, enable simultaneous imaging of hundreds of targeted intact proteins in a tissue or dispersion of cells. This eliminates the need for iterative cycling required for some high-plex fluorescence imaging platforms.

A key feature of these novel PCMTs is the ability to readily conjugate them to antibodies or other molecular probes in a 1-step high-yield chemical reaction. The resulting Miralys™ antibody probes consist of peptide mass-tags covalently attached through patented PC-Linkers to an antibody with an optional fluorophore for multimodal fluorescence and MALDI imaging on the same sample.

Miralys™ delivers the broadest spatial multiomic imaging in mass spectrometry

Spatial multiomic imaging reveals spatial patterns of biomolecules within tissues, helping scientists understand complex biological systems, uncover disease mechanisms, guide precision medicine, accelerate drug discovery, and foster biomedical innovation.

Miralys builds upon the already strong spatial multiomic capabilities of matrix-assisted laser-desorption/ionization (MALDI) imaging to add new layers of biological insight. Now, researchers can visualize over 100 intact targeted protein distribution in the same tissue sample in which they have already imaged unlabelled small molecule such as drugs, lipids and other metabolites.

Miralys delivers the broadest single instrument imaging capability

Miralys extends the multiomic capabilities of MALDI imaging allowing you to perform spatial multiomic imaging on the same sample with the same workflow, providing new insights.

Correlative Multimodal Imaging

Correlative Multimodal Imaging integrates two or more imaging techniques to capture diverse details about a single sample. This approach generates a more comprehensive profile of the specimen, offering insights into its macroscopic, mesoscopic, and microscopic characteristics, including its structure, dynamics, function, and chemical makeup. Mass Spec Imaging and Miralys MALDI-IHC are especially well-suited for use in multimodal workflows. Various imaging modalities can be performed either before or after MALDI-IHC imaging.

Miralys™ Probes

AmberGen’s patented probes are the heart of the Miralys imaging system.

Panels of Miralys Probes

Panels of Miralys probes are offered in either TurboPlex™ pre-mixed format, or VersaPlex™ fully configurable multi-vial format.

Unlike other technologies, Miralys™ Panels enable BOTH ultra-high-plex AND fast imaging.

Miralys Panels are ready-to-use curated collections of Miralys antibody imaging probes, designed and validated to reduce upfront assay development and validation time. The use of curated panels will significantly speed up your spatial discoveries.

Features

Maximum spatial multiomic data for a single tissue sample on one instrument platform
Inherently immune from autofluorescence and spectral overlap
Remarkably high plex and fast (up to 100+ biomarkers can be scanned in one hour for 1cm²of tissue)

Standard Miralys Probes

AmberGen maintains an inventory of standard probes that are validated. Download the MALDI HiPLEX-IHC Standard Probe list

Download Brochure

Dual-Label Miralys Probes

Our patented dual-label probes include both a photocleavable mass tag and a fluorophore that remains in place on the tissue after photocleavage. There are two dual-label probes on our standard probe list. Dual labelling is also an option for Custom Probes.

Miralys™ Kits

We understand how challenging it can be to onboard new technology. AmberGen provides a range of kits allowing you to conduct initial Miralys runs without using precious samples. Kits include reagent selection guidance, pre-staining and image review meetings, and a detailed Miralys workflow protocol.

Miralys™ Neuroscience Starter Kit

The neuroscience starter kit includes all the materials needed to implement the Miralys MALDI HiPLEX-IHC workflow in your lab, including validation slides and probe mix.

Kit contains:

A UV-Photocleavage light box that has been validated for use with our workflow.
A six-plex panel of probes; you have a choice between Neurology (mouse brain) or Immunology (human tonsil) kits.
Three FFPE tissue slides:
- - 1 slide is pre-stained with the six-plex Miralys probe mixture. This slide is provided for you to optimize your MALDI-MS imaging parameters.
  - 2 slides that are unstained. These are used for you to train on the slide immunohistochemical staining protocol. They are provided from the same tissue block.
All small laboratory items needed to run our protocol.

The purchase of a Miralys Starter Kit comes with a high level of support, including:

A “Pre-Staining” videoconference with an AmberGen Applications Scientist who will perform a walk-through of the protocol and provide tips and tricks to ensure the best possible experience.
A “Post-Staining” videoconference / image review where you can share your results and an AmberGen applications scientist will offer feedback and make suggestions for fine-tuning the variables of your staining and imaging process for future runs.
Email or telephone guidance as necessary.

Staining Protocol Practice Kit

The training slides and Miralys probe mix included in the Starter Kit are available for individual purchase. This is useful as a training tool for new lab employees learning the Miralys workflow. Kits contain:

A six-plex panel of probes; you have a choice between Neurology (mouse brain) or Immunology (human tonsil) kits
Three FFPE tissue slides:
- - 1 slide is pre-stained with the six-plex Miralys probe mixture. This slide is provided for you to optimize your MALDI-MS imaging parameters.
  - 2 slides that are unstained. These are used for you to train on the slide immunohistochemical staining protocol. They are provided from the same tissue block.

Antibody Conjugation Kit

The Miralys Antibody Conjugation Kit contains everything you need to create your own Miralys Probes. This includes a proprietary AmberGen photocleavable mass tag (PCMT) and all the necessary reagents and items needed to perform rapid one-step chemical conjugation of the PCMT to the antibody of your choice. More than 100 PCMTs are available for purchase to code the antibody of your choice. Each kit is designed to conjugate 100 µg of antibodies.

Contact us to discuss your needs

If you would like further information on AmberGen Miralys probes for mass spectrometry imaging, please fill in the form below and one of the team will be in touch.