The online coupling of electrochemistry with mass spectrometry has shown great potential for the investigation of drug metabolism in pharmaceuticals and for structure elucidation of biopharmaceuticals and proteins.
In proteomics and protein chemistry ROXY Reactors are mainly used for reduction of disulphide bridges for the assignment of disulphide bonds in biopharmaceuticals, and for adduct formation such as drug-protein binding.
In pharma, ROXY Reactors are mainly used for synthesis, and oxidative metabolism studies. Drug and xenobiotic metabolites are formed instantaneously in the electrochemical cell, mimicking the enzymatic biotransformation of the Cytochrome P450 reactions that usually take place in the liver (Phase I reaction). Hereby, Electrochemistry becomes a truly biomimetic tool for enzymatic REDOX reactions.
The ROXY™ Exceed is a new generation Potentiostat dedicated for coupling Electrochemistry with Mass Spectrometry (EC-MS). In on-line mode the ROXY Exceed can be used in direct infusion mode, for flow injection analysis, or with any type of LC-MS system. Applications and publications are available for using the ROXY in HDX-MS, top-down or bottom-up LC-MS proteomics. In off-line mode the ROXY Exceed is mainly used for electrochemical synthesis and for the collection of electrochemically generated products. Collected fractions are used for further analysis.
The Potentiostat is based on state-of-the-art electronics. It has a large voltage and current range of ± 4.9 V and 1 nA – 100 mA, respectively. It supports different modes of operation, DC, Scan, and Pulse for highest yields under the most challenging applications. The Exceed can be controlled via a contact closure from any LC-MS system by using Dialogue (Antec Scientific) or Chromeleon™ CDS (Thermo Fisher Scientific) software.
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