Focus of the MonthAntec Alexys Carbohydrate Analyser

Oligo– and Polysaccharides as Fraud Markers in Honey Carbohydrate Analysis

 

Honey is a popular consumer item as a natural sweetener.  However, the significant price difference between authentic honey and the cheaper sweeteners, such as sugar syrups, serve as a primary motivation for honey fraud through addition or blending. Parameters for determining honey fraud include levels of mono– and disaccharides in the honey, along with other parameters such as moisture content, diastase activity, and amino acid content.

 

This application note describes a method of carbohydrate analysis for the profiling of oligo– and polysaccharides in honey samples using High Performance Anion Exchange chromatography in combination with Pulsed Amperometric Detection (HPAE-PAD). The method is based on gradient separation of longer carbohydrate chains using the new SweetSep AEX200 high-resolution anion exchange column. As a proof of principle, several honey samples were analysed. In addition, the obtained carbohydrate profiles of an authentic honey sample were compared to those of intentionally adulterated honey sample to assess the method’s applicability in fraud detection.

 

Download the application note for more information: 220_028_02 Oligo-Polysaccharides-in-honey – https://verulamscientific.com/wp-content/uploads/2024/11/220_025_05-Carbohydrates-in-honey.pdf

 

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