HUDSON ROBOTICSLaboratory Workcells

Specifications

RapidHit Automated HitPicking System

Hudson’s RapidHit™ Simultaneous Automated HitPicking System is a compact, self-contained system that allows users to obtain assay plates comprised of the actual hits detected in a primary screen using the same compound source plates that provided the primary screen.  The RapidHit uses the output files produced by the plate reader as they become available, and automatically selects and transfers “hits” based on user-entered criteria.

As each primary screen assay plate is finished, the RapidHit immediately moves the hits to a secondary assay “hit plate”, and eliminates the possibility of receiving the wrong compounds in secondary screening plates.

The RapidHit saves the need to return to the user’s compound storage library to run secondary or conformational screens of the hits in a primary screen.  The hit-picked plates are prepared even while the primary screen is taking place, so the secondary or conformational screen can go forward without any delay.  And, because the original source compound plates are being used, the secondary assay uses the same compound conditions as the primary screen.  This eliminates any artifacts that may result from preparing secondary assay plates anew from the compound library.

ProLink Express – Proteomics Workcell

The ProLink Express is a fully automated proteomics workcell that integrates all microplate delivery and transfer operations with complete data tracking. It automates the entire protein expression process, from initial bacterial colony picking through to the final protein expression and measurement. The ProLink Express system can deliver a plate to any of its instruments from any other instrument, in any order and simultaneously with other processes. It can use a wide range of commercially available plasmid miniprep kits.

The ProLink Express integrates an automated colony picker with an incubator and a plasmid DNA miniprep system. A researcher can start with bacterial colony plates, isolate individual colonies into culture plate wells, grow the individual colonies and then extract the DNA plasmids in one seamless automated process without any human intervention. The system also provides the capability to re-attach DNA inserts onto expression vectors and perform in vitro or in vivo protein expression and detection.

Applications

• Protein Expression
• Gene Assembly
• Monoclonal Antibody Development and Production
• ELISAs

Features

• Optimizes laboratory processes and equipment usage
• Designed to fit into available lab space
• Allows independent as well as manual access to and operation of all major system instruments
• Capable of delivering a plate to any of its instruments from any other instrument, in any order and simultaneously with other processes
• Includes Hudson Robotics’s SoftLinx software, which provides an easy-to-use interface to coordinate the ProLink Express™ operation with other automated instruments; the software will enable the user to take full advantage of this system feature, which is unique in the lab automation industry.

Synthetic Biology Workstation

Hudson’s new Synthetic Biology Workstation automates the entire portion of the pipeline from Gene Assembly through Plasmid Preparation.

The following functions are automated and integrated into a single, easy to use system:

• Pipetting/Hit Picking
• DNA Normalization
• Transformations
• Plating Colonies
• Gene Assembly
• PCR
• Serial Dilutions
• Plasmid Prep

The software tracks everything from start to finish. DNA is normalized and transformations carried out. Agar is spread on Omni trays and colonies are plated to your specifications.

Applications

DNA and RNA Normalization

DNA Transformation into Bacteria

Gene Assembly

Microbiome/Microbiota Research

Next-Gen Sequencing (NGS)

Plasmid Preparation

Plasmid Prep Workcell

This small Plasmid Prep workcell supports standard miniprep protocols and includes a SOLO automated pipettor, a FilterPress automated 96 well filter plate positive pressure manifold, and a microplate shaking nest. High throughput can be added by including a PlateCrane robotic arm and microplate hotels. Hudson Robotics designs workcells for both commercially available plasmid miniprep kits as well as proprietary plasmid DNA miniprep protocols.

Applications

Plasmid Preparation

Applications

• DNA and RNA Normalization
• DNA Extraction
• Microbiome/Microbiota Research
• Oligo Pooling
• Oligo Purification
• Protein Expression & Purification
• Serial Dilutions

The extraction system has been designed to run commercial kits that extract DNA, RNA and proteins from a variety of sources including:

• Soil
• Plants
• Bacteria
• Blood
• Urine
• Saliva
• and more…

DNA Extraction Workstation (DNA, RNA and Proteins)

Hudson Robotics designs automated DNA extraction and RNA systems around your throughput requirements.

The DNA extraction and RNA extraction workstations greatly simplify the process of isolating pure nucleic acids and/or proteins from a wide variety of natural sources.

This space saving extraction system can be designed for labs with low throughput requirements where plates are manually loaded.

Higher throughput can be achieved using the PlateCrane EX™ or SciClops™ robot arm for hours of hands-off processing time. The workcells can utilize Hudson’s family of liquid handling instruments or 3rd party manufacturers liquid handling instruments or other instruments such as automatable centrifuges.

All instruments in the extraction workstation can be controlled by Hudson’s powerful SoftLinx automation software.

The system can be configured with any one, or all, of your favorite separation methods, including:

• vacuum filtration
• pressure filtration
• magnetic bead separation
• centrifuging

The utility of the extraction system can be greatly enhanced by adding additional equipment in range of the robot arm to add direct downstream processing of the purified bio-molecules.  Some examples of the potential versatility of combining the extraction system with additional equipment:

• Thermocycler: Direct amplification of the extracted and purified DNA (PCR).
• UV Spectrometer: Measure the concentration of purified proteins.
• Dynamic Light Scattering (DLS) reader: Measure the size and/or state of aggregation of each sample.

Contact Us

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