Focus of the MonthAntec Alexys Carbohydrate Analyser – Honey Fraud

Oligo–and polysaccharides as fraud markers in honey

 

Honey is a popular consumer item as a natural sweetener.  However, the significant price difference between authentic honey and the cheaper sweeteners, such as sugar syrups, serve as a primary motivation for honey fraud through addition or blending. Parameters for determining honey fraud include levels of mono– and disaccharides in the honey, along with other parameters such as moisture content, diastase activity, and amino acid content.

 

This application note describes a method for the profiling of oligo– and polysaccharides in honey sample using High Performance Anion Exchange Chromatography in combination

with Pulsed Amperometric Detection (HPAEC-PAD). The method is based on gradient separation of longer carbohydrate chains using the new SweetSep AEX200 high-resolution anion exchange column. As a proof of principle, several honey samples were analyzed. In addition, the obtained carbohydrate profiles of an authentic honey sample were compared to those of intentionally adulterated honey sample to assess the method’s applicability in fraud detection.

 

Click here to download the application note for more information.

 

Discover more about the Antec Alexys Carbohydrate Analysers: Electrochemical Detectors & Analysers | Antec Scientific (verulamscientific.com)

 

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