Focus of the MonthAntec Alexys Carbohydrate Analyser – Theory

The ALEXYS Carbohydrate Analyzer is based on High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD) using a gold working electrode. But what is PAD?

 

In pulsed amperometric detection (PAD), a rapid sequence of potential steps is applied. During a part of this ‘pulse sequence’, the signal is sampled. The other part of the sequence of steps, are oxidative and reductive cleaning steps. During this cleaning, the gold working electrode is regenerated. The process usually repeats about every half second. This ensures stable and reproducible detection conditions.

 

The use of PAD is necessary in carbohydrate analysis because oxidation results in insoluble reaction products that stick to the electrode surface. This contaminates the electrode and causes poor reproducibility. When using PAD with cleaning steps, the electrode remains clean and reproducible.

 

Discover more about the Antec Alexys Carbohydrate Analysers: Electrochemical Detectors & Analysers | Antec Scientific (verulamscientific.com)

 

Buy Antec consumables on our webshop: Electrochemical Detectors & Analysers | Antec Scientific (verulamscientific.com)

 

Or contact the Verulam Scientific team on 01234 381000 or email   [email protected]