Antec ScientificCarbohydrate Analysis

Learn more about our available instruments from Antec Scientific to perform effective carbohydrate analysis. Contact a member of the Verulam team today using the link below to place an order.

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Available Carbohydrate Analysis Instruments from Antec Scientific

ALEXYS Carbohydrate Analyser

The ALEXYS carbohydrate analyser uses High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD) for stable and highly sensitive carbohydrate analysis. High pH resistance and a maintenance-free HyREF reference electrode are achieved with specialised flow cells, FlexCell and SenCell. An inert gas blanketing kit for carbon dioxide-free mobile phase further enhances stable and sensitive analysis conditions.

The ALEXYS boasts detection limits as low as 10 nmol/L for carbohydrates, making it the optimal choice for analysing carbohydrates in:

Food
Beverages
Plants
Lactose-free products
Glycoproteins

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ET 210 Eluent Tray

High Performance Anion Exchange Chromatography (HPAEC) is used for HPLC carbohydrate analysis, a method that only works because carbohydrates become anions at pH 12 or higher. At such a high pH level, however, atmospheric CO2 forms carbonate ions in the mobile phase, which interfere with the column’s stationary phase, altering carbohydrate retention times and compromising reproducibility.

The ALEXYS eluent tray maintains an inert gas blanket over solvent bottles to prevent this issue and ensure consistent separation using:

Gas-tight caps
Specialised tubing
Fittings
Manometer

Why Choose Verulam Scientific?

Choosing Verulam Scientific for your analytical instrument requirements means choosing a supplier you can trust to deliver an unmatched level of service. We have carefully curated a team of highly qualified industry experts, allowing us to deliver customisable solutions, with new technologies regularly added to our extensive range. Whether you are looking for an individual unit or a complete system, we will work with you to find the ideal instruments and consumables to suit your needs. Learn more about how we operate by clicking the link below.

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What Our Customers Say

At Verulam Scientific, our commitment to delivering outstanding scientific instruments for our clients over the years has resulted in us becoming a trusted supplier for institutions across the UK. But don’t just take our word for it! Click the link below to learn more about what some of our previous customers have to say about a partnership with Verulam Scientific.

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FAQs

What Is Pulsed Amperometric Detection?

Pulsed Amperometric Detection (PAD) is a method used in HPLC carbohydrate analysis and related substances. This method of detection is necessary as these compounds tend to foul the surface of an electrode. Using PAD, carbohydrates can be oxidised and analysed at a high pH with high sensitivity in stable measurement conditions, ensuring reproducibility.

How Does Pulsed Amperometric Detection Work?

Pulsed Amperometric Detection (PAD) utilises a rapid sequence of potential steps. The signal is sampled during one part of this pulse sequence, while the other part consists of oxidative and reductive cleaning steps. This cleaning process regenerates the gold working electrode, ensuring stable and reproducible detection conditions. The entire process typically repeats approximately every half second.

Why Is the Use of PAD Necessary in Carbohydrate Analysis?

For the electrochemical detection of carbohydrates, a ‘pulse mode’ is required due to the tendency of oxidation products to adhere to the electrode surface. Pulsed Amperometric Detection (PAD) incorporates a cleaning step to ensure stable and reproducible detection.

Why Is Carbon Dioxide Removal Necessary in HPAEC?

HPAEC analyses carbohydrates via HPLC, requiring high pH levels for anionic separation. At these elevated pHs, atmospheric CO2 forms carbonate ions in the mobile phase, which alters carbohydrate retention times by occupying stationary phase functional groups. Efficient CO2 removal from the mobile phase is therefore crucial for reproducible separations. The ALEXYS eluent tray’s infrastructure (gas-tight caps, tubing, fittings, and manometer) maintains solvent bottles under a nitrogen or helium blanket, ensuring precise gas flow and minimal inert gas consumption.

How Can I Place an Order for a Carbohydrate Analysis Instrument?

Simply get in touch with a member of the Verulam team to place an order for an Antec instrument today. Browse the full range of Antec instruments online here.