The Miralys™ system from AmberGen Inc is designed to enable highly multiplexed imaging of high molecular weight biomolecules (such as proteins and glycans), traditionally difficult to analyse, in tissue samples using MALDI imaging. The MiralysTM system combines highly specific antibodies with the sensitivity and spatial resolution of MALDI imaging to enable the simultaneous detection of a multitude of biomarkers in a single tissue sample.
Read on to see how this is achieved
At the heart of AmberGen’s MALDI-IHC platform is its patented photocleavable mass-tags (PCMTs). These mass-tags act as barcodes, which when detected by a MALDI Mass Spectrometer, enable simultaneous imaging of hundreds of targeted intact proteins in a tissue or dispersion of cells. This eliminates the need for iterative cycling required for some high-plex fluorescence imaging platforms.
A key feature of these novel PCMTs is the ability to readily conjugate them to antibodies or other molecular probes in a 1-step high-yield chemical reaction. The resulting Miralys™ antibody probes consist of peptide mass-tags covalently attached through patented photocleavable-linkers to an antibody with an optional fluorophore for multimodal fluorescence and MALDI imaging on the same sample.
Miralys™ antibody probe staining of tissues is accomplished using standard manual or automated immunohistology protocols and equipment. After staining, the tissue is pre-UV photocleaved prior to matrix deposition and MALDI-MSI. Importantly, the photocleaved peptide mass-tag possesses a positively charged amino terminated ion which facilitates MS detection. Detection of each mass-tag ion produces a discrete peak at known m/z in the mass spectrum allowing reconstruction of 2D images of the spatial distribution of the targeted protein in the tissue slice.
Key Advantages
- HyperPlex Panels: Since hundreds of PCMTs can be used together, many ultra-high-plex panels can be created.
- Spatial Multiomic: Since it is based on MSI, both unlabelled small molecules such as metabolites and targeted macromolecules such as proteins can be imaged in the same tissue sample, on the same instrument.
- Spatial Multimodal: Dual-labelled fluorescence and mass-tag Miralys antibodies allow both fluorescence and MS imaging on the same sample using the same probes.
- Simultaneous Staining, Stability and Reimaging: All targeted biomarkers in a tissue are stained simultaneously. Stained slides can be stored for months for later imaging.
- Rapid Whole-Slide Imaging: Hundreds of PCMTs can be imaged simultaneously, meaning that hundreds of proteins can be imaged in a single scan.
Combine AmberGen MALDI-IHC platform with HTX matrix deposition MALDI Sprayers or SubliMATETM to elevate your MALDI imaging analysis.
Discover more about AmberGen MALDI-IHC platform: https://verulamscientific.com/ambergen/
Discover more about HTX MALDI Sprayers and sublimation: https://verulamscientific.com/htx-imaging-solutions/
Or contact the Verulam Scientific team on 01234 381000 or email [email protected]
