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LCTechSample Preparation of Mycotoxins
Customised Solutions
Customised Solutions
For mycotoxin analysis LCTech offer highly selective immunoaffinity columns (IAC), specific SPE columns or the unique CrossTOX® as a multitoxin column. All these columns can be used manually as well as in automation. The columns in SMART format are excellent for fast sample preparation with reduced solvent usage
Immunoaffinity Columns
The LCTech immunoaffinity columns are used successfully for a wide range of matrices.
– High matrix tolerance
– High loading capacity
– Matrix-optimised application protocols
– Can be automated by robotic system
AflaCLEAN™
The LCTech AflaCLEAN™ columns are based on immunoaffinity and are suitable for cleaning up aflatoxins B1, B2, G1, G2 in food and feed samples for HPLC and LC-MS/MS analysis. The antibodies used show a high specificity against the respective mycotoxin. The chromatographic results are outstanding, without any interfering signals and with very high recovery rates, even with difficult samples.
| Specifications | Details |
|---|---|
| Format | 3 mL |
| LOD (detection limit) | B1 0.030 ng/mL (ppt), B2 0.014 ng/mL, G1 0.012 ng/mL, G2 0.006 ng/mL |
| Validated matrices | Peanut, corn, rice, hazelnut, peppers, figs, dates, and more |
| Analyte | Aflatoxin B1, B2, G1, G2 |
| Storage | At 4°C to 30°C |
| Shelf life | 24 months from production date |
| Loading | 150 ng aflatoxin B1 |
| Recovery | B1 > 90 %, B2 > 80 % G1 > 90 %, G2 > 60 % |
| Packaging | 25 columns/pack or 500 columns/pack |
| Automation | FREESTYLE SPE |
AflaCLEAN™ SMART
SMART clean-up columns are particularly small. Solvent use and processing time are significantly reduced with these 3 cm, AOAC-compliant immunoaffinity columns.
| Specifications | Details |
|---|---|
| Format | SMART (length = 3 cm) |
| LOD (detection limit) | B1 0.007 ng/mL (ppt), B2 0.002 ng/mL, G1 0.008 ng/mL, G2 0.002 ng/mL |
| Validated matrices | Peanut, cinnamon, raisins, figs, paprika, rice, corn, pistachios, wheat, brazil nut |
| Analyte | Aflatoxin B1, B2, G1, G2 |
| Storage | At 4°C to 8°C |
| Shelf life | 9 months from production date |
| Loading | 100 ng aflatoxin B1 |
| Recovery | B1 ≥ 90 %, B2 ≥ 80 %, G1 ≥ 90 %, G2 ≥ 60 % |
| Packaging | 100 columns/pack, 1000 columns/pack |
| Automation | FREESTYLE ThermELUTE™ |
AflaCLEAN™ Select
LCTech AflaCLEAN™ Select are standard 3mL, low-cost version columns suitable for cleaning up aflatoxins B1, B2, G1, G2 in matrices such as peanut, maize, rice, paprika, figs, and more for HPLC and LC-MS/MS analysis.
| Specifications | Details |
|---|---|
| Format | 3 mL |
| LOD (detection limit) | B1 0.031 ng/mL (ppt), B2 0.015 ng/mL, G1 0.014 ng/mL, G2 0.007 ng/mL |
| Validated matrices | Peanut, maize, rice, paprika, figs and more |
| Analyte | Aflatoxin B1, B2, G1, G2 |
| Storage | At 4°C to 8°C |
| Shelf life | 9 months from production date |
| Loading | 200 ng aflatoxin B1 |
| Recovery | B1 ≥ 90 %, B2 ≥ 80 %, G1 ≥ 90 %, G2 ≥ 60 % |
| Packaging | 25 columns/pack 500 columns/pack |
| Automation | FREESTYLE SPE |
AflaCLEAN™ M1 Select
Standard version 3mL column for analysis of aflatoxin M1 in matrices such as milk, condensed milk, milk powder and other dairy products and baby food for HPLC and LC-MS/MS analysis.
| Specifications | Details |
|---|---|
| Format | 3 mL |
| LOD (detection limit) | 0.02 ng/mL (ppt) |
| Validated matrices | Milk, condensed milk, milk powder |
| Analyte | Aflatoxin M1 |
| Storage | At 4°C to 8°C |
| Shelf life | 12 months from production date |
| Loading | 100 ng aflatoxin M1 |
| Recovery | Aflatoxin M1 ≥ 90 % |
| Packaging | 25 columns/pack, 500 columns/pack |
| Automation | FREESTYLE SPE |
Afla-OtaCLEAN™
Standard version 3mL column for analysis of aflatoxins B1, B2, G1, G2 + ochratoxin A in food and feed matrices such as peanut, maize, rice, hazelnut, paprika, figs, dates for HPLC and LC-MS/MS analysis.
| Specifications | Details |
|---|---|
| Format | 3 mL |
| LOD (detection limit) | B1 0.025 ng/mL (ppt), B2 0.014 ng/mL, G1 0.012 ng/mL, G2 0.006 ng/mL, OTA 0.03 ng/mL |
| Validated matrices | Food and feed |
| Analyte | Aflatoxin B1, B2, G1, G2, ochratoxin A |
| Storage | At 4°C to 30°C |
| Shelf life | 18 months from production date |
| Loading | 150 ng aflatoxin B1, 200 ng ochratoxin A |
| Recovery | Aflatoxin B1 ≥ 90%, B2 ≥ 80% // G1 ≥ 90%, G2 ≥ 60% Ochratoxin A ≥ 90% |
| Packaging | 25 columns/pack, 500 columns/pack |
| Automation | FREESTYLE SPE |
AflaCLEAN™ M1 SMART for Aflatoxin M1
SMART columns with only 3 cm size for reduced solvent use suitable for matrices such as milk, milk powder, condensed milk and baby food. Compatible with EN ISO 14501:2007 and AOAC 2000.08. The SMART columns meet all criteria for compliant aflatoxin M1 control for dairy products.
| Specifications | Details |
|---|---|
| Format | SMART (length = 3 cm) |
| LOD (detection limit) | 0.0007 ng/mL (ppq) |
| Validated matrices | Milk, milk powder, condensed milk, baby food |
| Analyte | Aflatoxin M1 |
| Storage | At 4°C to 8°C |
| Shelf life | 9 months from production date |
| Loading | 100 ng aflatoxin M1 |
| Recovery | Aflatoxin M1 ≥ 80% |
| Packaging | 100 columns/pack, 1000 columns/pack |
| Automation | FREESTYLE ThermELUTE™ |
BioteX
Standard 3mL affinity columns for biotin analysis in baby food and vitamin supplements. High matrix loading suitable for HPLC and LC-MS/MS analysis
| Specifications | Details |
|---|---|
| Format | 3 mL |
| LOD (detection limit) | 1 ng/mL |
| Validated matrices | Baby food, vitamin supplements |
| Analyte | Biotin / vitamin B7 |
| Storage | At 4°C to 8°C |
| Shelf life | 9 months from production date |
| Loading | 1000 ng biotin |
| Recovery | > 90 % |
| Packaging | 25 columns/pack, 500 columns/pack |
| Automation | FREESTYLE SPE |
OtaCLEAN™
Standard 3mL immunoaffinity columns for ochratoxin A analysis in matrices such as cereals, dried fruits, nuts, coffee, spices. Suitable for HPLC and LC-MS/MS analysis.
| Specifications | Details |
|---|---|
| Format | 3 mL |
| LOD (detection limit) | 0,04 ng/mL (ppt) |
| Validated matrices | Cereals, dried fruit, nuts, coffee, spices |
| Analyte | Ochratoxin A |
| Storage | At 4°C to 30°C |
| Shelf life | 24 months from production date |
| Loading | 200 ng ochratoxin A |
| Recovery | Ochratoxin ≥ 90 % |
| Packaging | 25 columns/pack, 500 columns/pack |
| Automation | FREESTYLE SPE |
DONeX™
Standard 3mL immunoaffinity columns for deoxynivalenol analysis in matrices such as wheat, maize, barley, oats, cereals, animal feed. Suitable for HPLC and LC-MS/MS analysis.
| Specifications | Details |
|---|---|
| Format | 3 mL (SPE) |
| LOD (detection limit) | 1 ng/mL (ppt) |
| Validated matrices | Wheat, corn, barley, oats, cereals, feed |
| Analyte | Deoxynivalenol |
| Storage | No requirements |
| Shelf life | Unlimited |
| Loading | Up to 4 g matrix |
| Recovery | > 90 % |
| Packaging | 25 columns/pack, 500 columns/pack |
| Automation | FREESTYLE SPE |
OtaCLEAN™ SMART
AOAC-compliant immunoaffinity SMART columns ochratoxin A with only 3 cm size for reduced solvent use suitable for matrices such as coffee (green/roasted/soluble), rice, corn, dried fruits, beer, chilli, peppers and others. Suitable for HPLC and LC-MS/MS analysis.
| Specifications | Details |
|---|---|
| Format | SMART (length = 3 cm) |
| LOD (detection limit) | 0,01 ng/mL (ppt) |
| Validated matrices | Coffee (green/roasted/soluble), rice, corn, dried fruits, beer, chilli, paprika |
| Analyte | Ochratoxin A |
| Storage | At 4°C to 8°C |
| Shelf life | 9 months from production date |
| Loading | 100 ng ochratoxin A |
| Recovery | ochratoxin A ≥ 80 % |
| Packaging | 100 columns/pack, 1000 columns/pack |
| Automation | FREESTYLE ThermELUTE™ |
CrossTOX® for Multi Toxin Analysis
SMART columns with only 3 cm size for fast sample preparation for multi-mycotoxin analysis by LC-MS/MS – suitable for 18 toxins such as aflatoxins B/G OTA, DON, NIV, ZEA, FB1/FB2 and others. CrossTox columns can be used manually or automated fully with the FREESTYLE. Ideal for high-throughput laboratories.
| Specifications | Details |
|---|---|
| Format | SMART (length = 3 cm) |
| LOD (detection limit) | B1 0,04 ng/mL, B2 0,04 ng/mL, G1 0,04 ng/mL, G2 0,04 ng/mL, OTA 0,04 ng/mL, FB1 0,75 ng/mL, FB2 0,75 ng/mL, DON 0,1 ng/mL, ZEA 0,04 ng/mL, T-2 0,05 ng/mL, HT-2 0,05 ng/mL, Citrinin 0,1 ng/ mL, NIV 0,1 ng/mL, STC 0,1 ng/mL, DAS 1 ng/ mL |
| Validated matrices | Food (cereals, nuts, dried fruit), feed |
| Analyte | Aflatoxins B1, B2, G1, G2, sterigmatocystin, ochratoxin A, fumonisine B1 and B2, deoxynivalenol, nivalenol, 15-acetyl DON, 3-acetyl DON, DON-3-GLC, zearalenon, T-2 and HT-2, citrinin, diacetoxyscirpenol |
| Storage | Room temperature |
| Shelf life | 24 months from production date |
| Loading | 0.5 – 3 mL (analog to 0.6 g matrix) |
| Recovery | ≥ 85% |
| Packaging | 100 columns/pack, for manual use, 100 columns/pack, for automated use |
| Automation | FREESTYLE CrossTOX® |
ZeaCLEAN™ SMART
SMART columns with only 3 cm size for zearalenone analysis with reduced solvent use. Suitable for matrices such as such as grain, food, and feed for HPLC and LC-MS/MS analysis.
| Specifications | Details |
|---|---|
| Format | SMART (length = 3 cm) |
| LOD (detection limit) | 1 ng/ mL (ppt) |
| Validated matrices | Cereals, food, feed |
| Analyte | Zearalenone |
| Storage | Unlimited |
| Shelf life | 12 months from production date |
| Loading | Up to 0.5 g matrix |
| Recovery | Zearalenone ≥ 90 % |
| Packaging | 100 columns/pack, 1000 columns/pack |
| Automation | FREESTYLE ThermELUTE |
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