Affinity chromatography by His-tag is one of the most widespread purification techniques for recombinant proteins. In most cases there is an additional manual cleaning/polishing step. Use of the Knauer Azura FPLC allows quick and automated linkage of multiple chromatographic purification steps into one method. This eliminates manual sample handling and minimizes time spent between steps and simple handling errors.
This method can be adapted to suit your protein purification protocols.
VBS0067_Automated two step purification of 6xhis-tagged protein with AZURA BioLC_v2.pdf (knauer.net)
Discover more about KNAUER AZURA FPLC systems. Contact the @Verulam Scientific team on 01234 381000 or email [email protected]
Alternatively, check out our dedicated webpage | https://verulamscientific.com/knauer-chromatography-solutions/